Establecimiento de un protocolo para micropropagación in vitro de durazno (Prunus pérsica L.) var. Huayco rojo

Bertha Cecilia  Felix Tarazona; Jennifer Valeria  García Evangelista; Thaís Lusbenia  Quevedo Lázaro; Kevin  Hidalgo Quiroz; Yeltzin  Romero Torres; Mayra Xiomara  Tello Gallardo

doi:10.51431/bbf.v11i3.838

Authors

Bertha Cecilia Felix Tarazona Universidad Nacional José Faustino Sánchez Carrión https://orcid.org/0000-0001-7746-1292
Jennifer Valeria García Evangelista Universidad Nacional José Faustino Sánchez Carrión https://orcid.org/0000-0001-6768-9883
Thaís Lusbenia Quevedo Lázaro Universidad Nacional José Faustino Sánchez Carrión https://orcid.org/0000-0002-2082-1573
Kevin Hidalgo Quiroz Universidad Nacional José Faustino Sánchez Carrión https://orcid.org/0000-0003-0701-4870
Yeltzin Romero Torres Universidad Nacional José Faustino Sánchez Carrión https://orcid.org/0000-0003-0805-7944
Mayra Xiomara Tello Gallardo Universidad Nacional José Faustino Sánchez Carrión https://orcid.org/0000-0002-8298-3375

DOI:

https://doi.org/10.51431/bbf.v11i3.838

Keywords:

Micropropagation, prunus pérsica l., phytoregulators, in vitro

Abstract

Objective: Currently, peach production (Prunus persica L.) is one of the crops with the greatest demand for agroexport in Peru, which is why its production must be increased, with the in vitro micropropagation technique being one of the main ones. alternatives for its massiﬁcation. objective to standardize an efﬁcient method for the in vitro micropropagation of Prunus persica (Durazno) var. Red huayco from axillary buds. Methods: Young 2-month-old plants were used and pre-treated with fungicides and bactericides for disinfection. The axillary buds were introduced into MS medium vertically and incubated in bioclimatic chambers at 22°C and 24°C for 2 weeks each. Result: 75.1% of in vitro seedlings were obtained free of contamination and suitable for acclimatization under greenhouse conditions, 16.6% of contamination by bacteria and 8.3% of contamination by fungi were obtained. Conclusion: It is concluded that using the MS medium was an optimal medium for in vitro multiplication and using antioxidants for the oxidation of the axillary buds.